LOCUS       40924_2254        5009 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Boolean integrase logic vector NOR gate BCD-RFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 5009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..69
                     /label=P7
                     /note="P7"
                     /regulatory_class="promoter"
     misc_feature    70..122
                     /label=attB TP901-1
                     /note="attB TP901-1"
     terminator      complement(129..156)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(172..243)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    252..301
                     /label=attP TP901-1
                     /note="attP TP901-1"
     misc_feature    302..351
                     /label=attB Bxb1
                     /note="attB Bxb1"
     regulatory      352..464
                     /label=J61048
                     /note="J61048"
                     /regulatory_class="terminator"
     misc_feature    465..517
                     /label=attP Bxb1
                     /note="attP Bxb1"
     misc_RNA        519..569
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     misc_feature    593..677
                     /label=BCD2
                     /note="BCD2"
     RBS             663..671
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             675..1370
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     terminator      1393..1464
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1480..1507
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1514..1534
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1570..1601
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      1726..1820
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2312..2534
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2582..3529
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             3821..4678
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4692..4751
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      4811..4840
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      complement(4934..4963)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agaaaaaatt tatttgcttt cgcatctttt tgtacctata
       61 atgtgtggaa tgccaacaca attaacatct caatcaaggt aaatgctttt tgcttttttt
      121 gctataaacg cagaaaggcc cacccgaagg tgagccagtg tgactctagt agagagcgtt
      181 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      241 ttgatgcctg gaaaggagtt ttttagttac cttaattgaa ataaacgaaa taaaaactcg
      301 ctcggccggc ttgtcgacga cggcggtctc cgtcgtcagg atcatccggg ctttcttttg
      361 ggtatagcgt cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccga
      421 agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccgggggttt gtaccgtaca
      481 ccactgagac cgcggtggtt gaccagacaa accacgaagc tgtcaccgga tgtgctttcc
      541 ggtctgatga gtccgtgagg acgaaacagc ctctacaaat aattttgttt aagggcccaa
      601 gttcacttaa aaaggagatc aacaatgaaa gcaattttcg tactgaaaca tcttaatcat
      661 gctaaggagg ttttctaatg tcagaattaa ttaaagaaaa tatgcacatg aaattatata
      721 tggaaggtac tgtcaacaat catcatttca aatgcacatc cgaaggtgaa ggtaaaccat
      781 atgaaggcac acaaacaatg cgcatcaaag cagttgaagg tggacccctg ccctttgcgt
      841 ttgacattct cgcaacgagc tttatgtacg ggtctaaaac ttttatcaat cacacccaag
      901 gcattcctga cttttttaaa cagtcctttc ctgaaggctt tacctgggaa cgtgtaacaa
      961 cttatgaaga tggcggtgta cttacagcaa ctcaagatac gagtttacaa gatggctgtc
     1021 tgatttacaa tgttaaaatc cgtggcgtaa atttcccgag taacggaccc gtaatgcaaa
     1081 aaaaaactct tggttgggaa gcatcaacag aaaccttata tcctgcggac ggtggcttag
     1141 aaggacgcgc agacatggca ctgaaattag ttggaggcgg tcatttaatc tgcaacctga
     1201 aaacaaccta tcgttccaaa aaacccgcta aaaaccttaa aatgcctgga gtatactatg
     1261 ttgatcgtcg cttagaacgt attaaagaag ctgataaaga aacctacgtt gaacaacatg
     1321 aagtagccgt agcccgttat tgtgaccttc cgtcgaaatt aggacatcgt tgataatact
     1381 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1441 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1501 ttctgcgttt atatactagt agcggccgct gcaggagtca ctaagggtta gttagttaga
     1561 ttagcagaaa gtcaaaagcc tccgaccgga ggcttttgac taaaacttcc cttggggtta
     1621 tcattggggc tcactcaaag gcggtaatca gataaaaaaa atccttagct ttcgctaagg
     1681 atgatttctg ctagagctgt cagaccaagt ttacgagctc gcttggactc ctgttgatag
     1741 atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     1801 cgttttttat tggtgagaat ccaagcacta gggacagtaa gacgggtaag cctgttgatg
     1861 ataccgctgc cttactgggt gcattagcca gtctgaatga cctgtcacgg gataatccga
     1921 agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag tcagatagca
     1981 ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt ttcttgtatt
     2041 atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt acccccattc
     2101 actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg actcaggtgc
     2161 ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga gggtgctact
     2221 taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt gcgacatcct
     2281 tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg gatctattct
     2341 ttttatcttt ttttattctt tctttattct ataaattata accacttgaa tataaacaaa
     2401 aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta caagttttcc
     2461 agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga catgtaatta
     2521 tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc aattgagatg
     2581 tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct atgacttaac
     2641 ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca cgattgaaaa
     2701 ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgctc agatgatgaa
     2761 catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc tgatgacgag
     2821 aactgtggaa atcaggaatc ctttggttaa aggctttgag attttccagt ggacaaacta
     2881 tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat tgccttatct
     2941 tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt ttgaaaacaa
     3001 atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga aaactcacaa
     3061 ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg aaaataacta
     3121 ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag atttaaacac
     3181 ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata cgttgatttt
     3241 ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca accagataaa
     3301 aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac gtaacggact
     3361 aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt ttgaggcaaa
     3421 atttttgagt gacatgcaaa gtaagcatga tctcaatggt tcgttctcat ggctcacgca
     3481 aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct gaggttctta
     3541 tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga acaactgttc
     3601 accgttagat atcaaaggga aaactgtcca tatgcacaga tgaaaacggt gtaaaaaaga
     3661 tagatacatc agagctttta cgagtttttg gtgcatttaa agctgttcac catgaacaga
     3721 tcgacaatgt aactactaga gctaaataca ttcaaatatc tatccgctca tgagacaata
     3781 accctgataa atgcttcaat aatattgaaa aaggaagaat atgagtattc aacatttccg
     3841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3901 gctggtgaaa gtaaaagatg ccgaagatca gttgggtgca cgtgtgggtt acatcgaact
     3961 ggacctcaac agcggtaaga ttcttgagag ttttcgcccc gaagaacgtt tcccaatgat
     4021 gagcactttt aaagttctgc tctgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4141 agaaaagcat cttacggacg gcatgacagt acgcgaatta tgcagcgctg ccataaccat
     4201 gagtgataac acggcggcca acttacttct gacaacgatc ggaggaccga aggagcttac
     4261 cgcttttttg cacaacatgg gtgatcatgt aactcgcctt gatcgttggg aaccggagct
     4321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcta tggcaacaac
     4381 gttgcgcaaa ctcttaactg gcgaacttct tactctcgct tcccggcaac aattaataga
     4441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4501 gtttattgct gataaatctg gagccggtga gcgtgggtcc cgcggtatta ttgcagccct
     4561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gccaggcaac
     4621 tatggacgaa cgtaatcgcc agatcgctga gataggtgcc tccctgatta agcattggta
     4681 ataatactag ctccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa
     4741 aaccgaaaag attacttcgc gtttgccacc tgacgtctaa gaaaaggaat attcagcaat
     4801 ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta
     4861 attagttagt tagcccttag tgactctgcc acctgacgtc taagaaaagg aatattcagc
     4921 aatttgcccg tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac
     4981 gtaattagtt agttagccct tagtgactc
//
